Fig. 6: Binding affinities of human ACE2 with different spikes and the cell entry of pseudoviruses. | Nature Communications

Fig. 6: Binding affinities of human ACE2 with different spikes and the cell entry of pseudoviruses.

From: Bat and pangolin coronavirus spike glycoprotein structures provide insights into SARS-CoV-2 evolution

Fig. 6

a Binding curves of immobilized hACE2 with the SARS-CoV-2, PCoV_GX, or RaTG13 spike. Data are shown as different colored lines and the best fit of the data to a 1:1 binding model is shown in black. b The cell entry efficiencies of pseudoviruses as measured by luciferase activity. SARS-CoV-2, PCoV_GX, and RaTG13 pseudoviruses were used to infect hACE2-transfected HEK 293 T cells. Data shown are from three independent experiments. Data are presented as mean values ± SEM. c The representative micrographs and 2D classification results of negative-staining EM. Both spikes were incubated with 4-fold molar ratio of hACE2. The red boxes show the complex of the PCoV_GX spike with hACE2.

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