Fig. 2: Target expression is preserved in human metastases and upon subcutaneous or orthotopic transplantation. | Nature Communications

Fig. 2: Target expression is preserved in human metastases and upon subcutaneous or orthotopic transplantation.

From: Identification of CD318, TSPAN8 and CD66c as target candidates for CAR T cell based immunotherapy of pancreatic adenocarcinoma

Fig. 2

a Target candidate staining of three independent primary PDACs and matched metastases. Cell nuclei were stained with DAPI (blue), tumor cells by cytokeratin (green), marker of interest (MOI) is depicted in red. P1 and P3 metastases are of lymph node origin, P2 is liver derived. Images are representative for one stained sample. b Flow cytometry staining of target candidates on dissociated PAXF1872 PDX cells transplanted either orthotopically (blue) or subcutaneously (yellow). Respective isotype control is indicated in red. Scale bar = 150 µm for all tissues except for P2, metastasis, CD318 = 300 µm. P = patient. TSPAN8 staining of primary PDAC from P3 not available due to technical failure.

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