Fig. 6: SPAD values and metal concentrations in the newest leaves of rice plants supplied with chelating agents.
From: Development of a mugineic acid family phytosiderophore analog as an iron fertilizer
a SPAD values were measured 7 days after a single application at 4 days after transplantation. b–e Metal concentrations in the newest leaves. Ten pots were used in each treatment, and three rice plants were grown in each pot. The three newest leaves in each pot were collected and used for measurement of SPAD values and metal concentrations by inductively coupled plasma optical emission spectrometry. The colors of the plots of each treatment through a–e indicate samples from the same pots. Values are means with standard deviations indicated by error bars (n = 10 for control, Fe-EDDHA and Zn-EDTA, n = 9 for PDMA, Fe-PDMA, and Fe-EDTA, and n = 8 for Zn-PDMA, after excluding outliers of Fe concentration), in which each replicate is either average of three plants in each pot for a, or total metal concentration of the three plants in each pot for b–e. Different letters indicate significant differences at P < 0.05 by Tukey’s honestly significant difference test (two-sided). PDMA proline-2′-deoxymugineic acid, EDDHA ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid), EDTA ethylenediaminetetraacetic acid, SPAD soil and plant analyzer development, D.W. dry weight.
