Fig. 4: ICS responses in CD4⁺ and CD8⁺ T cells for M and NP pools in ELISpot positive individuals.

ICS was performed on individuals with convalescent mild cases and a positive ELISpot for the indicated peptides. PBMC were stimulated with 2 μg/ml peptide for 6 h. Expression levels of IFN-γ, IL-2 and TNF in CD4⁺ and CD8⁺ T cells using M pools are shown in a, n = 31. Bars represent median ± IQR. Statistics were performed using a two-tailed Wilcoxon matched-pairs signed-rank test between each cytokine in CD4⁺ vs CD8⁺ T cells. Boolean gates were then set and cytokine expression was examined in CD4⁺ T cells (n = 9) using SPICE (b). Error bars represent SEM for cytokine expression figures. Expression levels of cytokines using NP pools are shown in c (n = 41) with polyfunctionality analysis for CD4⁺ T cells (d) (n = 4) as above.