Fig. 1: Exposure to hyperoxia induced an arrest in alveolarization in the developing mouse lung.
From: Single cell transcriptomic analysis of murine lung development on hyperoxia-induced damage
a Mouse pups were exposed from day of birth to room air (21% O2, gray) or hyperoxia (85%O2, blue). A total of 36 lungs were harvested on postnatal days (P)3, 7, and 14. n = 6/group. b Representative histological sections from lungs developing in 21% O2 (black circles) or 85% O2 (purple squares) at P3, P7, and P14. Seven animals/group were evaluated. Scale bar = 200 µm. c Body weight was assessed at P3, P7, and P14. Lung volume was assessed by Archimedes principle at P7 and P14. Precise measurements at P3 were not possible due to the small size of the organ. Lung morphometry was quantified by the mean linear intercept (MLI) measurement. Data are presented as means ± SD. Statistical analyses were performed with GraphPad Prism 8.0. The presence of potential statistical outliers was determined by Grubbs’ test. Significance was evaluated by multiple unpaired Student’s t-test with Holm–Sidak correction. P values **** = <0.0001. n = 7 animals/group.
