Fig. 5: SSNMR spectra of uniformly and sparsely 13C-labeled mouse RIPK3 fibrils highlighting some long-range inter-residue correlation peaks.
From: The amyloid structure of mouse RIPK3 (receptor interacting protein kinase 3) in cell necroptosis
a 2D 13C–13C correlation spectrum of sparsely 13C-labeled mouse RIPK3 fibrils using [2-13C]-labeled glycerol with 200 ms DARR mixing, showing the carbonyl region. The long-range correlation peak L456Cβ/Cγ-Q449Cδ are highlighted. b 2D 13C–13C correlation spectrum of uniformly 13C-labeled mouse RIPK3 fibrils with 500 ms DARR mixing, showing the aromatic region. The dashed lines indicate the correlation peaks of F442, and the solid lines indicate the correlation peaks of Y453. c 13C–15N TEDOR correlation spectrum of sparsely 13C-labeled mouse RIPK3 fibrils using [2-13C]-labeled glycerol with 6.4 ms z-filtered TEDOR recoupling time. The protein is also uniformly 15N-labeled. TEDOR shows the correlation peaks of N454Nδ2-G451Cα and Q449Nε2-L456Cγ. d 2D 13C–13C correlation spectrum of sparsely 13C-labeled mouse RIPK3 fibrils using [1,3-13C]-labeled glycerol with 500 ms DARR mixing. The assignment of V441Cγ-G451Cα is unambiguous.
