Fig. 4: Osmotic stability of P. falciparum-infected normocytes.

a Developmental stage of in vitro-cultured, cryopreserved P. falciparum (clone 3D7 P2G12) (n = 5). Bars represent the mean ± SEM. Inset are representative images of P. falciparum IDC development stages. Scale bar, 10 μm. Gray (stage I), green (stage II), blue (stage III), red (stage IV), yellow (stage V) bars indicate IDC development stage. b P. falciparum-infected RBC lysis₅₀ values normalized to lysis₅₀ values of corresponding uninfected RBCs at 24- and 44-h of in vitro culture of cryopreserved P. falciparum (clone 3D7 P2G12) (n = 5). Unique symbols indicate biological replicates. Horizontal lines and error bars represent mean ± SEM. n.s., no significant difference between P. falciparum-infected RBC lysis₅₀ values at 24 and 44-h of culture (p = 0.1) using paired two-tailed Student’s t-test. c Lysis curves of 24-h (gray dotted line) (n = 7) and 44-h (gray solid line) (n = 6) cryopreserved Brazilian P. vivax-infected reticulocyte cultures and 24-h (black dotted line) (n = 5) and 44-h (black solid line) (n = 5) cryopreserved P. falciparum-infected normocytes (clone 3D7 P2G12) cultures. Unique symbols indicate biological replicates. Data fit with least-squares regression fit curves of normalized data.