Fig. 2: Deficiency in METTL3 protects mice against VSV infection in vivo.
a Survival of Mettl3f/f Lyz2-Cre and Mettl3f/f littermate female mice (n = 5 per group) at various times (horizontal axes) after intraperitoneal infection with VSV (1 × 109 PFU per mouse). b, c Microscopy of hematoxylin-and-eosin-stained lung (b) or liver (c) sections from female mice treated with PBS or VSV. d qRT-PCR analysis of Ifnb1 and VSV mRNA in the spleens (left), livers (middle), and lung (right) of Mettl3f/f Lyz2-Cre and Mettl3f/f littermate female mice (n = 5 per group) given intraperitoneal injection of PBS or infected for 24 h by intraperitoneal injection of VSV (2.5 × 108 PFU per mouse); results are presented relative to those of actin. e Western blot analysis of VSV-G in the spleens, livers, and lungs of infected mice. f ELISA analysis of IFN-βin serum. n = 6 biologically independent animals. g Isolated peritoneal macrophages from VSV-infected mice, and then performed Western blot by indicated antibodies. h qRT-PCR analysis of Mettl3 conditionally knocked out in hepatocytes. n = 3 biologically independent experiments. i Survival of Mettl3f/fAlb-Cre and Mettl3f/f littermate female mice (n = 5 per group) at various times (horizontal axes) after intraperitoneal infection with VSV (1 × 109 PFU per mouse). j, k qRT-PCR analysis of Ifnb1 (i) and VSV (j) mRNA in the spleens, livers, and lung of Mettl3f/f Alb-Cre and Mettl3f/f littermate female mice (n = 5 per group) given intraperitoneal injection of PBS or infected for 24 h by intraperitoneal injection of VSV (2.5 × 108 PFU per mouse); results are presented relative to those of actin. l ELISA analysis of IFN-β in serum. n = 5 biologically independent animals. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 as determined by two-tailed unpaired Student’s t test (d, f, h, j–l). Log-rank (Mantel–Cox) test (a, i). Error bars represent mean ± SEM.
