Fig. 4: METTL3 mediates m6A modification on VSV RNA. | Nature Communications

Fig. 4: METTL3 mediates m6A modification on VSV RNA.

From: N6-methyladenosine RNA modification suppresses antiviral innate sensing pathways via reshaping double-stranded RNA

Fig. 4

a A schematic representation of the experimental procedure used in b. b Dot blot analysis of VSV RNA m6A level treated with immunoprecipitated (IP)-IgG or IP-METTL3, respectively. Methylene blue staining indicated the loading control. c MeRIP-qPCR analysis of VSV RNA m6A level following treatment with overexpression of WT or Mettl3-mut, respectively. n = 2 biologically independent experiments. d RNA-FISH analysis of the co-localization of VSV RNA and m6A modification in the cytosol. e The conserved sequence motif of m6A residues in CIMS-based miCLIP-seq. f Integrative genomics viewer (IGV) plots of the METTL3-binding regions and m6A modification on VSV negative sense (−) genomic RNA (Upper graph) and VSV positive sense (+) RNAs (Lower graph). m6A sites are indicated by red triangles. g MeRIP-qPCR analysis of specific m6A sites on VSV RNA in WT or Mettl3 KO RAW264.7 cells. n = 2 biologically independent experiments. *p < 0.05, **p < 0.01, as determined by two-tailed unpaired Student’s t test (c, g). Error bars represent mean ± SEM.

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