Fig. 5: METTL3-mediated m6A modification reshapes viral dsRNA. | Nature Communications

Fig. 5: METTL3-mediated m6A modification reshapes viral dsRNA.

From: N6-methyladenosine RNA modification suppresses antiviral innate sensing pathways via reshaping double-stranded RNA

Fig. 5

a Immunofluorescent analysis of dsRNA level after PBS treatment or VSV infection for 12 h in shNC and shMETTL3 HeLa cells. n = 10 cells examined over 2 independent experiments. b Dot blot analysis of dsRNA level after VSV infection for 12 h in HeLa cell. Methylene blue staining indicates equal RNA loading. The bar graph shows the statistics (right). n = 3 independent experiments. c, d Immunoblot (c) and dot blot (d) shows the immunoprecipitated (IP)-IgG or IP-dsRNA, and the m6A level in the IP-dsRNA in shScr and shMETTL3 HeLa cells. Methylene blue staining indicates equal RNA loading. The bar graph shows the statistics (right). e Anti-dsRNA-RIP-qPCR analysis of the dsRNA level in VSV RNA in shScr and shMETTL3 HeLa cells. The bar graph (Right panel) shows the statistics from the mean value of each m6A site (Left panel). Data are representative of 2 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 as determined by two-tailed unpaired Student’s t test (a, b, d, e-left) or two-tailed paired Student’s t test (e-right). Error bars represent mean ± SEM.

Back to article page