Fig. 4: MAIT polyfunctionality evaluated after in vitro stimulation in PBMC of melanoma patients.
From: Circulating mucosal-associated invariant T cells identify patients responding to anti-PD-1 therapy
A Left and center panels: representative dot plots of MAIT cells, identified as TCR 7.2+ and CD161+ within CD8+ T cells of one R and one NR at T0. Right part: proportion of MAIT cells in R and NR at T0, T1, T2. Data represent individual values, mean (center bar) ± SEM (upper and lower bars). Statistical analysis by two-sided Mann–Whitney nonparametric test, Bonferroni’s multiple comparisons test. *p = 0.016. NR = 4, R = 8. B Left panel: UMAP representation of PBMC or tumor-infiltrating lymphocytes from patients with metastatic melanoma. Expression of KLRB1, CD69, and CXCR4 MAIT cells in blood (B), lymph nodes metastasis (LN), and Tumor (T) from the K383, K409, and K411 patients (Gene Expression Omnibus, GSE148190). Right panel: UMAP representation with the distribution of cells of B, LN and T, and proportion of MAIT in blood (B), lymphnode (LN) and tumor (T). C Left and central panels: pie charts representing the proportion of MAIT cells producing different combinations of GRZM-B, IFNγ, and TNF after stimulation at T0. Frequencies were corrected by background subtraction as determined in unstimulated controls; permutation tests (10,000 number of permutations), using SPICE software, show no difference between R and NR. Right panel: frequency of MAIT cells expressing and producing different combinations of GRZM-B, IFN-γ, and TNF after stimulation at T0. Statistical analysis by Wilcoxon rank test; *p = 0.041. In the figure, error bars (median value) and upper whiskers (whisker range, SEM) are represented. Individual measurements of NR = 4, R = 6. D Left and central panels: dot plots show the difference between a R and a NR in the proportion of cells that produce IFN-γ and GRZM-B at T0. Right part, proportion of MAIT cells producing GRZM-B at different time points. Data represent individual values, mean (center bar) ± SEM (upper and lower bars). *p = 0.028, Statistical analysis by Mann–Whitney nonparametric test, Bonferroni’s multiple comparisons test. Individual measurements of NR = 4, R = 6. Source data are provided as a Source Data file.
