Fig. 1: Cardiomyocyte-specific overexpression of Xbp1s mitigates cardiac steatosis and induces FoxO1 protein degradation in HFpEF. | Nature Communications

Fig. 1: Cardiomyocyte-specific overexpression of Xbp1s mitigates cardiac steatosis and induces FoxO1 protein degradation in HFpEF.

From: Xbp1s-FoxO1 axis governs lipid accumulation and contractile performance in heart failure with preserved ejection fraction

Fig. 1

a Representative images of Oil Red O staining of left ventricular (LV) sections from wild-type (WT) and cardiomyocyte-restricted Xbp1s overexpressing (Xbp1s TG) HFpEF mice. Scale bars = 50 μm. Images are representative of four hearts/group. b Cardiac triglyceride content in myocardial tissue from WT and Xbp1s TG control (CTR) and HFpEF hearts (n = 4/group). c Representative immunoblot images of FoxO1 and GAPDH proteins from LV of WT and Xbp1s TG mice under CTR and HFpEF conditions. Images are representative of three hearts/group. d Densitometric analysis of FoxO1 protein band. (n = 3/group). e Representative immunoblot images of FoxO1 and GAPDH proteins from neonatal rat ventricular myocytes (NRVMs) transduced with adenovirus expressing green fluorescent protein (GFP; AdGFP) or Xbp1s (AdXbp1s). Images are representative of four independent experiments. f Densitometric analysis of FoxO1 protein band in the different experimental groups. (n = 4 biologically independent experiments). g Representative immunoblot images of FoxO1, Lamin A/C, and GAPDH proteins in cytosolic and nuclear extracts from NRVMs transduced with AdGFP or AdXbp1s. Images are representative of four independent experiments. h Densitometric analysis of cytosolic and nuclear FoxO1 protein band in the different experimental groups (n = 4 biologically independent experiments). i Representative immunoblot images of FoxO3, FoxO1, Xbp1s, and GAPDH proteins from NRVMs transduced with AdGFP, AdXbp1s, or not transduced (−) in presence or absence of MG132. Arrow indicates FoxO3-specific band. Images are representative of five independent experiments. j Densitometric analysis of FoxO1 protein band in the different experimental groups (n = 5 biologically independent experiments). Results are presented as mean ± S.E.M. In b, d, and j, *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001, two-way ANOVA plus Sidak’s multiple comparisons test. In f and h, *p < 0.05, unpaired, two-tailed Kolmogorov–Smirnov test.

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