Fig. 5: STUB1 mediates Xbp1s-dependent FoxO1 protein degradation. | Nature Communications

Fig. 5: STUB1 mediates Xbp1s-dependent FoxO1 protein degradation.

From: Xbp1s-FoxO1 axis governs lipid accumulation and contractile performance in heart failure with preserved ejection fraction

Fig. 5

a Representative immunoblot images of FoxO1, STUB1, and GAPDH proteins from NRVMs transfected with siSTUB1 or scrambled siRNA control and treated with cycloheximide (CHX) for different durations (0, 1, 4, and 8 h). Images are representative of five independent experiments. b Densitometric analysis of FoxO1 protein band in the different experimental groups. (n = 5 biologically independent experiments). c mRNA level of FoxO1 in NRVMs transfected with siSTUB1 or scrambled siRNA control and transduced with AdGFP or AdXbp1s (n = 4 biologically independent experiments). d Representative immunoblot images of FoxO1, Xbp1s, STUB1, and GAPDH proteins from NRVMs transfected with siSTUB1 or scrambled siRNA control and transduced with AdGFP, AdXbp1s. Images are representative of four independent experiments. e Densitometric analysis of FoxO1 protein band in the different experimental groups. (n = 4 biologically independent experiments). Results are presented as mean ± S.E.M. In b, c, and e *p < 0.05, **p < 0.005, ****p < 0.0001, two-way ANOVA plus Sidak’s multiple comparisons test.

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