Fig. 3: PGC1 is required for CM hypertrophy and contractility development.
From: PGC1/PPAR drive cardiomyocyte maturation at single cell level via YAP1 and SF3B2
a Experimental scheme showing generation and analyses of a cmKO heart achieved by injection of AAV9-cTnT-Cre into PGC1α/βflox/flox; Ai9 mice at p0. b Heart slice showing a cmKO myocyte in the myocardium (top) and dissociated control (middle) and cmKO (bottom) myocytes. c Violin plots of cell area distributions in control (blue) and cmKO (red) CMs at p7 (p-value = 0.003), p14 (p-value = 2.5e−7), p28 (p-value = 2.2e−16). n = 44, 11, 90, 49, 522, 132 (left to right). ANOVA followed by post hoc Bonferroni tests were used. d–f Sarcomere shortening data with the average trace, fractional shortening (p-value = 0.047) and contraction velocity (p-value = 0.07). Control n = 13, cmKO n = 19. The student’s t-test was used. g–i Calcium handling with average calcium trace, peak height (p-value = 0.023, AU = arbitrary units), and time to baseline 90 (p-value = 0.0006). Control n = 13, cmKO n = 9. Mann–Whitney–Wilcoxon test and the Student’s t-test were used. Box-and-whiskers plot represents the maxima, 75th percentile, median, 25th percentile, and minima. P-value: *<0.05,**<0.01,***<0.001,****<0.0001.
