Fig. 2: CHD4 regulates PADI1 and PADI3 expression and citrullination of their substrates. | Nature Communications

Fig. 2: CHD4 regulates PADI1 and PADI3 expression and citrullination of their substrates.

From: Citrullination of pyruvate kinase M2 by PADI1 and PADI3 regulates glycolysis and cancer cell proliferation

Fig. 2

a, b PADI1 and PADI3 expression in the indicated cells lines following CHD4 silencing shown by RNA-seq (a) and RT-qPCR (b). n = 3 biological replicates. and unpaired t-test with two tailed p-value analyses and confidence interval 95% were performed by Prism 5. p-Values: *p < 0.05; **p < 0.01; ***p < 0.001. Data are mean ± SEM. c Volcano plot showing proteins with increased or decreased total PSMs after immunoprecipitation with pan-citrulline antibody. Increases in number of total and citrullinated PSMs in CHD4 silenced cells is shown below the plot. d Enrichment for a collection of glycolytic enzymes following CHD4 silencing. e Immunoblot showing expression of recombinant PAD1 and PAD3 in cells transfected with the corresponding expression vectors of the empty vector (EV). f Immunoblot showing expression of PKM2 in cells after CHD4 silencing or transfection with the PAD1 and PAD3 vectors in the cell extracts used for immunoprecipitation with pan-citrulline antibody. g PKM2 in the pan-citrulline IPs from 501Mel or MM117 cells. h. Immunoblot showing PKM2 in the pan-citrulline IP after transfection with the PADI1 and/or PADI3 expression vectors.

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