Fig. 7: PKM2 citrullination contributes to increased glycolysis in hypoxia. | Nature Communications

Fig. 7: PKM2 citrullination contributes to increased glycolysis in hypoxia.

From: Citrullination of pyruvate kinase M2 by PADI1 and PADI3 regulates glycolysis and cancer cell proliferation

Fig. 7

a–c Correlation of PADI1 and PADI3 expression with that of the HARRIS_HYPOXIA signature in the indicated cancer types using TCGA data sets. The Spearman correlations and p-values are indicated. d, e Expression of the indicated genes assessed by RT-qPCR in cells grown under hypoxia (1% O2) (d) or pseudo-hypoxia with 300 μM DMOG (e). n = 3 biological replicates. f Induction of PAD1 and PAD3 expression in 501Mel cells grown as 3D melanospheres for 7 days. g Immunoblots showing enhanced PKM2 R106Cit when grown under increasing concentrations of DMOG. h Immunoblots showing expression of the indicated proteins in transfected cells in presence of 300 μM DMOG. i Glycolysis in transfected cells in presence of 300 μM DMOG. ECAR values were determined from n = 4 biological replicates with 6 technical replicates for each N and unpaired t-test with two tailed p-value analyses and confidence interval 95% were performed by Prism 5. p-Values: *p < 0.05; **p < 0.01; ***p < 0.001. Data are mean ± SEM.

Back to article page