Fig. 1: Identification of inhibin-β-A as a gene highly expressed in lung microvasculature.

a Genes whose expression in MVECs-L was three times greater than in MVECs-D, CAECs, or PAECs were separately identified through the DNA microarray analysis. Venn diagram analysis of these genes was shown. Numbers indicate the number of genes included in the sets of the intersections. We focused on genes whose expression in MVECs-L was three times greater than in all of MVEC-Ds, CAECs, and PAECs (the center intersection surrounded by red box). The heat map of these particular genes was shown. Arrow indicates INHBA. b Genes whose expression in the lung was five times greater than in the heart, kidney, or liver were separately identified through the DNA microarray analysis. Venn diagram analysis of these genes was shown. Numbers indicate the number of genes included in the sets of the intersections. We focused on genes whose expression in the lung was five times greater than in all of the heart, kidney, and liver (the center intersection surrounded by red box). The heat map of these particular genes was shown. Arrow indicates INHBA. c, d Quantitative PCR analysis for inhibin-β-A (INHBA) (b), and inhibin-β-B (INHBB), and inhibin-α (INHA) (c) in ECs and SMCs isolated from various vascular beds (n = 4 biologically independent cells in each group). HPAEC human pulmonary artery EC, HCAEC human coronary artery EC, HMVEL-D human microvascular EC in dermis, HPASMC human pulmonary artery SMC. Data are presented as the mean ± SEM.