Fig. 1: Identification of α1AT as SARS-CoV-2 inhibitor.
From: Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection
a Caco2 cells were treated with peptide/protein containing fractions of the bronchoalveolar lavage library (or EK1 peptide as inhibitor control) and transduced with luciferase-encoding lentiviral SARS-CoV-2 spike pseudoparticles. b Caco2 cells were treated with subfractions of mother fraction 42 (see a) of the bronchoalveolar lavage library and transduced with lentiviral SARS-CoV-2 spike pseudoparticles. Blue columns represent pseudoparticle entry and black line absorbance at 280 nm of the corresponding fraction. Transduction rates in a and b were determined 2 days after the addition of pseudoparticles by measuring luciferase activities in cell lysates. The means ± SEM from n = 2 (a) or individual datapoints from n = 1 (b) independent experiments are shown, each performed in biological duplicates. The active fraction 42_55 (red box) was analyzed via gel electrophoresis, gel excision, tryptic digest, and MALDI-TOF-MS to identify α1AT, a serine protease inhibitor (serpin). Source data are provided as a Source data file.
