Fig. 2: α1AT inhibits SARS-CoV-2 spike but not VSV-G mediated pseudovirus entry. | Nature Communications

Fig. 2: α1AT inhibits SARS-CoV-2 spike but not VSV-G mediated pseudovirus entry.

From: Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection

Fig. 2

a Prolastin (α1AT) and the control small molecule inhibitor camostat mesylate (CM) were added to Caco2 cells 1 h prior to transduction of cells with rhabdoviral SARS-CoV-2 spike (blue) or VSV-G pseudoparticles (gray). b Caco2 cells were treated for the indicated hours with Prolastin (α1AT) and were then transduced with rhabdoviral SARS-CoV-2 spike pseudoparticles. c Prolastin (α1AT) was added 2 h post transduction of Caco2 cells with rhabdoviral SARS-CoV-2 spike pseudoparticles. Transduction rates in a–c were determined at 16 h after addition of pseudoparticles by measuring luciferase activities in cell lysates. The mean ± SEM from n = 2 experiments in biological triplicates are shown (2-way ANOVA with Dunett´s multiple comparison test). Source data are provided as a Source data file.

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