Fig. 2: Combinatorial assembly of gene expression controllers.
From: Harnessing the central dogma for stringent multi-level control of gene expression
a Summary of the 8-part genetic template used to allow for systematic exploration of direct and multi-level gene regulation. The 4-bp overhangs used for Golden Gate assembly are shown in grey at their respective junctions. Available genetic elements are listed below each corresponding part type (A–H). b The MLC toolkit contains a set of plasmids that can be combined using Golden Gate assembly to create a variety of direct and multi-level controllers (Supplementary Fig. 3). c The lacI transcription factor responsive to IPTG used for level 1 (L1) transcriptional regulatory control. d Toehold switch (THS) translational regulator used for level 2 (L2) control. e Small transcription activating RNA (STAR) transcriptional regulator used for L2 control. f Dual control (DC) transcriptional and translational regulator used for L2 control.
