Fig. 3: Performance comparison of single- and multi-level controllers in vivo.
From: Harnessing the central dogma for stringent multi-level control of gene expression
a Total GFP fluorescence for ‘off’ and ‘on’ input states (0 and 1 mM IPTG, respectively). Points show the three biological replicates for each controller and condition (black circles, Ptac; blue squares, THS; red diamonds, STAR; orange crosses, DC). Black dashed line denotes the mean fluorescence of cell autofluorescence (a.f.) controls containing no plasmid with grey shaded region showing ±1 standard deviation of 11 biological replicates. Fluorescence given in calibrated molecules of equivalent fluorescein (MEFL) units. b Flow cytometry distributions of total GFP fluorescence for ‘off’ (line) and ‘on’ (shaded) input states. Cell autofluorescence (a.f.) controls containing no controller are shown by black dashed line and light grey filled distributions. c Doubling time of cells harbouring direct and multi-level controllers for varying concentrations of IPTG (bars left to right for each design: 0, 0.1, 1, 10 mM IPTG). d Lag time calculated as the time to reach an OD600 = 0.15 after inoculation of cells harbouring controllers for varying concentrations of IPTG (bars left to right for each design: 0, 0.1, 1, 10 mM IPTG). Source data are provided as a Source Data file.
