Table 1 Performance summary of the single- and multi-level controllers in vivoa.

From: Harnessing the central dogma for stringent multi-level control of gene expression

Controller

Typeb

Basalc (%)

Dynamic ranged (103 MEFL)

Fold changed

Co-operativitye, n

SNRf (dB)

Ptac

SLC

0.5

0.9

93

3.4

0.2

THS

MLC

0.02

65.5

2166

7.3

10.1

STAR

MLC

1.45

4.6

53

9.3

4.7

STAR2

MLC

2.0

3.4

37

7.7

4.5

DC

MLC

0.04

10.4

1030

3.4

7.1

  1. aAll values are averages calculated from three biological replicates. Key performance features of the controllers are visually shown in Supplementary Fig. 1.
  2. bSLC refers to ‘single-level controller’ and MLC refers to ‘multi-level controller’.
  3. cRelative basal expression calculated when no IPTG is present and as a percentage of the expression level for the ‘on’ state (1 mM IPTG).
  4. dCalculated between ‘on’ and ‘off’ states for cells grown in 0 and 1 mM IPTG, respectively, and given in calibrated molecules of equivalent fluorescein (MEFL) units.
  5. eFrom the Hill function fitting of the steady-state response functions (Fig. 4a).
  6. fSNR refers to ‘Signal to Noise Ratio’.
Back to article page