Table 3 Acquisition parameters for the various experimental configurations.

From: Simultaneous spatiotemporal super-resolution and multi-parametric fluorescence microscopy

Camera

Analysis

Laser wavelength (nm)

Time per frame (ms)a

No. of frames

Image pixel size (nm)

Map dimensions (Pixels)

EMCCD

FCS

488

2

50,000

240

46 × 46

Fig. 2

SRRF

488

200

500

24

460 × 460b

EMCCD

FCS

561

2

50,000

240

120 × 56

Fig. 4

Diffusion law

561

2

50,000

240

114 × 56

N&B

561

20

5,000

240

120 × 56

SRRF

488

200

500

48

600 × 280c

  1. aAcquisition time was 2 ms; longer times are reached by time binning of frames.
  2. b,cThe original image has a pixel size of 120 nm (b) and 240 nm (c) at 1 × 1 binning, respectively. During the SRRF analysis, virtual sub-pixels of sizes 24 nm (b) and 48 nm (c), respectively, are created.