Fig. 8: Electron immunomicroscopic localization of Hax-1 in Purkinje cells (PC) using diaminobenzidine labeling to localize immunoreactivity. | Nature Communications

Fig. 8: Electron immunomicroscopic localization of Hax-1 in Purkinje cells (PC) using diaminobenzidine labeling to localize immunoreactivity.

From: Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1

Fig. 8

a, b Immunostaining detected at the plasma membrane of wild-type animals. c, d Hax-1 immunoreactivity at sites where mitochondria are apposed to the plasma membrane in wild-type animals. e Hax-1 on endoplasmic reticulum (wild-type mice). f Hax-1-immunoreactive protrusions at the soma of a neuron from a wild-type animal. g Hax-1 at the plasma membrane in G592R Kv3.3 mice. h Mitochondrial/plasma membrane Hax-1 immunoreactivity in G592R Kv3.3 mice. i and j Hax-1 on endoplasmic reticulum in G592R Kv3.3 mice. k and l Examples of multivesicular bodies with internal Hax-1 immunoreactivity in G592R Kv3.3 animals. Hax-1 immunostaining is representative of 40 and 32 images taken from sections of three wild type and three G592R Kv3.3 mice, respectively. Scale bars for all figures, 500 nm.

Back to article page