Fig. 4: Truncation of bL27 causes hypersensitivity to acylaminooxadiazoles. | Nature Communications

Fig. 4: Truncation of bL27 causes hypersensitivity to acylaminooxadiazoles.

From: trans-Translation inhibitors bind to a novel site on the ribosome and clear Neisseria gonorrhoeae in vivo

Fig. 4

A Growth of E. coli ∆tolC expressing full-length bL27 (black) or variants missing residues 2–4 (-3, blue) or 2–7 (-6, red) was monitored in broth microdilution experiments and the IC50 for MBX-4132 was determined. At least two technical replicates were performed for each biological replicate and the average of at least three biological replicates is shown with error bars indicating standard deviations. B Violin plot showing normalized mean IC50 values from at least 3 biological replicates of experiments as in (A) show that truncation of bL27 increases sensitivity to KKL-35 and MBX-4132 but has no effect on erythromycin (ERM), linezolid (LZ), spectinomycin (SPEC) or tetracycline (TET). Source data are provided as a Source Data file.

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