Table 1 Cryo-EM data collection, refinement and validation statistics.
|  | 70S • P-site tRNA • KKL-2098 (EMDB-20121) (PDB 6OM6) |
|---|---|
Data collection and processing | Â |
  Magnification | 59,000 |
  Voltage (kV) | 300 |
  Electron exposure (e–/Å2) | 58 |
  Defocus range (μm) | 1.5–3.5 |
  Pixel size (Å) | 1.191 |
  Symmetry imposed | C1 |
  Initial particle images (no.) | 474,382 |
  Final particle images (no.) | 28,121 |
  Map resolution (Å) | 3.1 |
   FSC threshold | 0.143 |
  Map resolution range (Å) | 3.1 |
Refinement | Â |
  Initial model used (PDB code) | 5MDV |
  Model resolution (Å) | 3.1 |
    FSC threshold | 0.143 |
  Model resolution (Å) | 3.3 |
  Map sharpening B factor (Å2) | 88.04 |
 Model composition |  |
    Non-hydrogen atoms | 144295 |
    Protein residues | 5715 |
    Ligands | KKL: 1 |
 B factors (Å2) | min/max/mean |
    Protein | 96.44/652.55/167.47 |
    Ligand | 152.66 |
 R.m.s. deviations |  |
    Bond lengths (Å) | 0.007 |
    Bond angles (°) | 0.897 |
 Validation |  |
    MolProbity score | 1.73 |
    Clashscore | 6.66 |
    Poor rotamers (%) | 0.3 |
 Ramachandran plot |  |
    Favored (%) | 94.7 |
    Allowed (%) | 4.22 |
    Disallowed (%) | 1.12 |
