Fig. 2: ASXL1-MT expands the pLT-HSC compartment along with dysregulated hematopoiesis during aging.

a Enumeration of white blood cells (WBC), hemoglobin (Hb), and platelets (Plt) in peripheral blood of aged Vav-Cre ASXL1-MT KI mice (n = 13 (Control) and 12 (ASXL1-MT)). b The frequency of myeloid cells (CD11b⁺), B cells (B220⁺), and T cells (CD3⁺) in peripheral white blood cells of aged Vav-Cre ASXL1-MT KI mice (n = 13 (Control) and 12 (ASXL1-MT)). c The frequency of LSK cells, MPPs, ST-HSCs, and LT-HSCs in bone marrow cells of young and aged Vav-Cre ASXL1-MT KI mice (n = 6 (Young) and 5 (Aged)). Representative FACS plot (left panel) and summarized data (right panel) are shown. d The experimental design for competitive transplantations. 200 LT-HSCs isolated from aged Vav-Cre ASXL1-MT KI mice and age-matched control mice were transplanted into lethally irradiated recipient mice with 4 × 10⁵ whole bone marrow cells. e Levels of donor chimerism in peripheral blood were analyzed at the indicated weeks after transplantation (n = 7 (Control) and 8 (ASXL1-MT)). f 6 months after transplantation, the frequency of donor-derived cells in peripheral blood (PB), whole bone marrow cells, Lin⁻ cells, Lin⁻c-kit⁺Sca1⁻(LK) cells, MPPs, ST-HSCs, and LT-HSCs were analyzed (n = 6 (Control) and 8 (ASXL1-MT)). Data are mean ± s.e.m. Data are mean ± s.d. unless otherwise noted. Data are assessed by two-tailed Student’s t-test (a, b, e, f) or one-way ANOVA with Tukey–Kramer’s post-hoc test (c). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.