Fig. 3: ASXL1-MT confers a fitness advantage on LT-HSCs with aging. | Nature Communications

Fig. 3: ASXL1-MT confers a fitness advantage on LT-HSCs with aging.

From: Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway

Fig. 3

a The experimental design for partial induction of ASXL1-MT in vivo using Mx1-Cre ASXL1-MT KI mice. Young Mx1-Cre ASXL1-MT KI mice were partially induced to express ASXL1-MT by pIpC injections (10 μg/mouse × 5 times) at 12 weeks after birth. 18 months after pIpC injections, bone marrow cells from Mx1-Cre ASXL1-MT KI mice were analyzed. b The frequency of ASXL1-MT expressing cells in peripheral blood at the indicated months after pIpC injections (n = 20). c Enumeration of white blood cells (WBC), hemoglobin (Hb), mean corpuscular volume (MCV), and platelets (Plt) in peripheral blood of Mx1-Cre ASXL1-MT KI mice at the indicated months after pIpC injections (n = 20). d The frequency of LSK cells, MPPs, ST-HSCs and LT-HSCs in ASXL1-MT induced cells, ASXL1-MT non-induced cells, or whole bone marrow cells of age-matched control mice (n = 9). Representative FACS plot (left panel) and summarized data (right panel) are shown. e Cell cycle analysis with Ki-67/DAPI staining of LT-HSCs of aged Mx1-Cre ASXL1-MT KI mice and age-matched control mice (n = 6). f The frequency of ASXL1-MT-expressing cells in peripheral blood, whole bone marrow, LSK, MPP, ST-HSC, and LT-HSC fractions of aged mice (18 months after pIpC injections) (n = 9). Data are mean ± s.e.m. Data are assessed by two-tailed Student’s t-test (b, c, e) or one-way ANOVA with Tukey–Kramer’s post-hoc test (d). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.

Back to article page