Fig. 5: The enhanced sensory perception upregulates AMPK activity through par-4 and octopamine.
From: The decrease of intraflagellar transport impairs sensory perception and metabolism in ageing
a PAR-4 (green) is expressed in neurons, including sensory neurons. Similar results were obtained in all independent experiments. The endogenous par-4 gene was tagged with mNeonGreen (mNG) using CRISPR/Cas9 technology. DYF-1::TagRFP-T (magenta) marks sensory neurons. Scale bar: 10 μm. b The ciliary localisation of PAR-4::GFP (arrows) is enhanced by overexpressing daf-19c. Scale bar: 5 μm. n = 66 and 58 animals, respectively. p = 0.0233. c The neuron-specific RNAi against par-4 blocks the upregulation of p-AMPK in worms overexpressing daf-19c. n = 3 biological independent experiments. Exact p value are included in Source Data file. d tdc-1 and tbh-1 are two genes encoding critical enzymes in octopamine synthesis. e The levels of p-AMPK in the indicated worms. Worms were collected at day 1 of adulthood post 30 min of 4 mM octopamine or mock treatment. n = 3 biological independent experiments. Exact p values are included in Source Data file. α-tubulin serves as loading controls in western blot assays. Data are presented as mean ± SEM in c and mean ± SD in the rest. Unpaired t-test (two-tailed) in b, c, one-way ANOVA in e, *p < 0.05, **p < 0.01, ***p < 0.001, ns non-significant. Source data are provided as a Source Data file.
