Fig. 2: m⁶A methylation on COOLAIR is required for FCA-mediated FLC silencing.

a Expression of unspliced FLC relative to UBC in C2 and fip37 C2 2-week-old seedlings, both carrying transgene pLEC1::FIP37. Data are normalized to C2. Data are mean ± s.e.m. from three biological replicates. P value from two-tailed t test. b Expression of unspliced and spliced FLC relative to UBC in C2 and mta C2 (carrying transgene pABI3::MTA) plants. Data are normalized to C2. Data are mean ± s.e.m. from three biological replicates. P value from two-tailed t test. c m⁶A-IP-qPCR analyzing m⁶A enrichment on chromatin-associated COOLAIR transcript after immunoprecipitated by m⁶A antibody. a–g represent amplicons over COOLAIR in qPCR analysis, with positions indicated on the top schematic. Data are mean ± s.d. from five to six biological replicates. d LC-MS/MS determined m⁶A-to-A ratio on nuclear RNA immunoprecipitated by FCA, with and without RNase T1 digestion. Data are mean ± s.d. from six biological replicates. Two-tailed P value from multiple t test corrected by Holm–Sidak method. Source data are provided as a Source Data file.