Fig. 3: m⁶A enhances FCA binding to COOLAIR and FCA condensates formation.

a FCA–RIP-qPCR analyzing FCA binding to COOLAIR transcript in mta mutant and the corresponding wild-type control (CTL). x axis represents the amplicons in qPCR (positions refer to the schematic in Fig. 2d). Data are mean ± s.d. from three biological replicates. Two-tailed P value from multiple t test corrected by Holm–Sidak method. b Representative images of root tip nuclei expressing pFCA::FCA-mTurquoise2 in plants with and without mta mutation. Maximum intensity projections of Z-stack spanning the entire width of a nucleus were applied. Scale bars, 5 μm. c Quantification of FCA-mTurquoise2 condensates number in root cells in plants with and without mta mutation. Data were plotted from minima to maxima. The box extends from the 25th to 75th percentiles. The line inside the box marks the median. The whiskers go down to the minima and up to the maxima. P value from two-tailed t test. d, The distribution of FCA-mTurquoise2 condensates of different sizes (in two groups, size between 0.01 and 0.02 μm² and bigger than 0.02 μm²) in plants with (n = 59 nuclei) and without (n = 60 nuclei) mta mutation. P = 0.0237, two-sided Fisher’s exact test. e The ratio of proximal-to-distal isoforms of COOLAIR transcripts (refer to the schematic in Fig. 2d) in mta C2 relative to corresponding wild-type C2. Data are mean ± s.d. from three biological replicates. f DRIP–qPCR analyzing COOLAIR R-loop in mta mutant and corresponding wild-type control (CTL). Data are mean ± s.d. from three biological replicates. Source data are provided as a Source Data file. Raw and processed images for Fig. 3c, d are available from Figshare (https://doi.org/10.6084/m9.figshare.13645730.v1).