Fig. 1: Mitochondria–lysosome contacts dynamically form in human neurons. | Nature Communications

Fig. 1: Mitochondria–lysosome contacts dynamically form in human neurons.

From: Dysregulation of mitochondria-lysosome contacts by GBA1 dysfunction in dopaminergic neuronal models of Parkinson’s disease

Fig. 1

a Representative 3D structured illumination microscopy (N-SIM) images of M–L contacts (yellow arrows) in wild-type human dopaminergic iPSC-derived neurons (mitochondria: red, Mito–RFP; lysosomes: green, Lyso-GFP) (30 neurons from N = 3 independent experiments were imaged). b Representative electron microscopy (EM) images of M–L contacts (yellow arrows) with distance between membranes <10 nm (mitochondria, M; lysosomes, L). c Representative time-lapse confocal images of dynamic contacts between mitochondria (red, Mito-RFP) and lysosomes (green, Lyso-GFP). Time-lapse recordings were taken at 2 s intervals for 3–5 min. Yellow arrows mark stable M–L contacts. White arrows mark the site of M–L contacts before contact formation or after contact untethering. Black line shows duration of contacts (see Supplementary Movies 1 and 2). d, e Quantification of duration of stable M–L contacts from confocal images (n = 156 contacts from 26 neurons, N = 3 independent experiments). d Average minimum duration of neuronal M–L contacts. e Relative frequency (percentages) distribution of neuronal M–L contacts. X-axis represents bin centers. Bin width = 60 s. f Percentage of lysosomes contacting mitochondria (for >20 s) (n = 41 neurons, N = 3 independent experiments). For all quantifications, data are means ± S.E.M. Scale bars, 500 nm (a, c); 100 nm (b).

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