Fig. 2: Glutathione and N-acetylcysteine impairs oligomerization of secreted cystatin C L68Q.
From: NAC blocks Cystatin C amyloid complex aggregation in a cell system and in skin of HCCAA patients
Supernatants were incubated in the presence of the indicated concentrations of glutathione or NAC for 1 h at 37 °C. Samples were mixed with 2% SDS without reducing agents prior to electrophoresis, and protein levels were detected by anti-cystatin C antibody and amounts of monomeric hCC (Mono) or high-molecular- weight hCC species (HMW) were quantitated. No HMW fraction was detected in supernatants from HEK-293T cells stably expressing hCC WT (N = 3 independent experiments; one-tailed student t test; indicated p values were less than 0.05, without correction for multiple testing, with respect to untreated). Bars are means, error bars represent standard deviation. Gel images for replicate experiments are shown in Supplementary Fig. 3.
