Fig. 2: Performance of TBD25AG as a trifunctional reporter/selector.
From: Robust and flexible platform for directed evolution of yeast genetic switches
a Schematic illustration of Tet-ON-controlled TBD25AG gene expression. Nucleosides (5FdU or dP) are incorporated via hENT1 and are toxified by the kinase activity of TBD25AG. Zeocin exerts antibiotic activity towards cells, which is alleviated by TBD25AG expression. The expression level of TBD25AG can be quantified by GFP fluorescence. b–d Functions of TBD25AG. Chromosomal expression of TBD25AG under control of the Tet-ON system was measured by b cellular GFP fluorescence, c cell viability in Zeocin-containing medium, or d cell viability in the 5FdU-containing medium. Error bars represent the mean ± SD of three independent experiments. Source data are available in the Source Data file.
