Fig. 2: Identification of IEL CD8+ T cell and CD4+ T cell subsets by flow cytometry.

a Schematic t-SNE clustering of IEL CD8+ T cells. Number illustrates the relationship with clusters observed by scRNA-seq. Asterisk illustrates the different experimental approach. U; undefined. b t-SNE clustering of IEL CD8+ T cells from a representative donor showing expression of CD103, CD39, and KLRG1. c Schematic t-SNE clustering of IEL CD4+ T cells. Number illustrates the relationship with clusters observed by scRNA-seq. Asterisk illustrates the different experimental approach. d t-SNE clustering of IEL CD4+ T cells from three representative donors showing expression of CD161, CD39, GP130, TIGIT, BTLA, PD-1, ICOS, and CD25.