Fig. 2: SARS-CoV-2 infection increases mTORC1 activity. | Nature Communications

Fig. 2: SARS-CoV-2 infection increases mTORC1 activity.

From: SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition

Fig. 2

a mTORC1 activity is increased in SARS-CoV-2 infected Vero kidney epithelial cells. Protein was extracted from Vero cells infected with SARS-CoV-2 for 24 h and immunoblotted for the indicated proteins (representative of two biological independent samples). b mTORC1 activity is increased in ALI mucociliary cultures derived from two different donors. Protein was extracted from ALI cultures infected with SARS-CoV-2 for 72 h and immunoblotted for the indicated proteins (n = 2 biological independent samples). c pAKT and pERK are increased in SARS-CoV-2 infected NHBE ALI cultures (representative of two biological independent samples). d mTORC1 activity is increased in COVID-19 patient lungs. IHC staining of p4EBP1 and the macrophage marker CD68 on lungs from COVID-19 and non-COVID-19 patients. Images are representative of two out of five COVID-19 patient lungs and nine out of nine non-COVID-19 lungs. Red arrows indicate cells positive for p4EBP1. Scale bar 50 µM.

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