Fig. 7: Changes in the differential exonic usage of SMIM11A mRNA in young (20–34 years, n = 12) compared to old (80+ years, n = 5) age groups.

a Red and blue bars represent the average exon usage for all 17 exons of SMIM11A mRNA in the young and old groups, respectively. DEXSeq analysis identified one exonic region (E011) that significantly changed in abundance between the age groups (pink in the gene model). The average exonic usage in this region is higher in the young age group and lower in the old age group. Of the seven variants of SMIM11A mRNA, two have statistically significant changes in expression continuously with age according to our linear model. Linear model p-values (two-sided Wald tests, unadjusted) are displayed for these variants. The horizontal axis below the variant models denotes the positions in the genome. b Ages of participants in the young (red) and old (blue) age groups are shown on the vertical axis. UCSC browser tracks indicate that the exons displaying differential expression (location on each transcript identified by red bar) identified in our DEXSeq analysis are more expressed in the young age group than in the old age group, indicated by the presence of larger blue peaks in younger participants. UCSC browser tracks support our DEXSeq findings.