Fig. 7: 6-TG abrogates toxin production by reducing transcription from the agr locus and from genes encoding for ribosomal proteins.

a RNA-seq analysis of wild-type S. aureus cultures treated with a vehicle control or 6-TG (10 µg/mL) in TSB. Data points above the dashed lines along the x-axis represent p ≤ 0.01. Data points outside of the dashed lines along the y-axis represent fold changes of ≥2.0. Results are from four independent experiments. b RNA-seq analysis of S. aureus purK::ΦΝΣ cultures treated with a vehicle control compared to wild-type S. aureus cultures treated with a vehicle control in TSB. Results are from four independent experiments. c RNA-seq analysis of S. aureus purK::ΦΝΣ cultures treated with a vehicle control or 6-TG (10 µg/mL) in TSB. Results are from four independent experiments. d RNA-seq analysis of wild-type S. aureus cultures and S. aureus purK::ΦΝΣ cultures treated with 6-TG (10 µg/mL) in TSB. Results are from four independent experiments. Statistical significance was determined using a negative binomial distribution. Detailed RNA-seq data can be found in Supplementary Data 2.