Fig. 2: Specific knockdown of unc13A and unc13B in ePNs.
From: Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila
a Confocal images from the calyx (upper) and LH (lower) regions in animals expressing UAS: unc13A-RNAi (unc13A-knockdown) or UAS: unc13B-RNAi (unc13B-knockdown) or both (double-knockdown) under control of GH146-Gal4, compared with heterozygous driver line, GH146 (control), immunostained against Unc13A (magenta) and Unc13B (green). Scale bars: 10 μm. b, c Expression level of Unc13A and Unc13B proteins in calycal boutons (b) and lateral horn (c) regions in animals expressing UAS: unc13A-RNAi (unc13A-knockdown) or UAS: unc13B-RNAi (unc13B-knockdown) or both (double-knockdown) under control of GH146-Gal4, compared with heterozygous driver line, GH146 (control). The values show a significant reduction of Unc13A in unc13A-knockdown group (green plot) compared with the control group (gray plot) while no change was observed for Unc13B level. The level of Unc13B significantly reduced in unc13B-knockdown group (red plot) compared with the control group (gray plot) while no change was observed for Unc13A level. The level of both Unc13A and Unc13B significantly reduced in the double-knockdown group (blue plot) compared with the control group (gray plot). Control group: n = 10, unc13A-knockdown: n = 9, unc13B-knockdown: n = 10, double-knockdown: n = 9 (b). Control group: n = 10, unc13A-knockdown: n = 8, unc13B-knockdown: n = 10, double-knockdown: n = 9 (c). P values were calculated via one-way ANOVA with the Bonferroni multiple comparison post hoc test (n.s., not significant (p > 0.05), ***p ≤ 0.001). d–e Confocal (lower) images of Unc13A (d) or Unc13B (e) immunostaining and time-gated STED (gSTED; upper) images of BRP Nc82 (green) and Unc13B (d) or Unc13A (e) (magenta) at MB calyx. Scale bars: 200 nm. f The Unc13B nano-distribution relative to the BRP ring center is unchanged in unc13A knockdown (green plot) group in comparison with the control gray (gray plot). Similarly, unc13B knockdown (red plot) does not significantly alter the distance between Unc13A and the BRP ring center. P values were calculated via two-tailed Mann–Whitney U test (n.s., not significant (p > 0.05), n = 5 brains, 24–30 synapses per brain). Values represent mean ± SEM. Source data including the exact sample sizes and the p values are provided as a Source Data file. See also Supplementary Fig. 3.
