Fig. 3: Both, Unc13A and Unc13B, specifically contribute to synaptic transmission from ePN output synapses.
From: Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila
a–o In vivo calcium imaging of homer-GCaMP3 using two-photon microscopy at postsynaptic terminals of MB Kenyon cells in the calyx upon odor stimulation, 4-methylcyclohexanol (a–e), benzaldehyde (f–j) and 2, 3-butanedione (k–o). a, f, k Representative false color-coded two-photon images of odor-induced fluorescence changes of GCaMP3 in MB calyx (mb247: homer-GCaMP3) at the dendritic terminals of Kenyon cells. b, g, l Odor-induced fluorescence change (ΔF/F0 %) of homer-GCaMP3 in animals expressing UAS: unc13A-RNAi (unc13A-knockdown, green traces) or UAS: unc13B-RNAi (unc13B-knockdown, red traces) or both (double-knockdown, blue traces) under control of GH146-Gal4 was compared with the control group (GH146-Gal4/+; mb247: homer-GCaMP3/+, gray traces). c, h, m Maximum fluorescence changes of GCaMP3 (Max ΔF/F0 %) upon odor stimulation. The Max ΔF/F0 (%) in unc13A-knockdown (green), unc13B-knockdown (red) and double-knockdown (blue) shows a significant reduction compared to the control group (gray). d, i, n The ΔF/F0 (%) traces of the first second of odor stimulation for 4-methylcyclohexanol (d) benzaldehyde (i), and 2, 3-butanedione (n). e, j, o Time to maximum fluorescence changes of GCaMP3 (time to peak) upon odor stimulation. The time to peak (ms) is significantly increased in unc13A-knockdown (green) and double-knockdown (blue) groups compared with the control group while no change was observed for unc13B-knockdown group (red). Values represent mean ± SEM. Control group: n = 11, unc13A-knockdown: n = 12, unc13B-knockdown: n = 8, double-knockdown: n = 10 (b–e). Control group: n = 11, unc13A-knockdown: n = 10, unc13B-knockdown: n = 9, double-knockdown: n = 9 (g–j). Control group: n = 13, unc13A-knockdown: n = 12, unc13B-knockdown: n = 10, double-knockdown: n = 10 (l–o). All p values were calculated via one-way ANOVA with the Bonferroni multiple comparison post hoc test (n.s., not significant (p > 0.05), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). Source data including the exact sample sizes and the p values are provided as a Source Data file. See also Supplementary Fig. 4 and 5.
