Fig. 3: Inhibition of miR-135a-5p induces memory impairments in vivo. | Nature Communications

Fig. 3: Inhibition of miR-135a-5p induces memory impairments in vivo.

From: miR-135a-5p mediates memory and synaptic impairments via the Rock2/Adducin1 signaling pathway in a mouse model of Alzheimer’s disease

Fig. 3

a Diagram of the AAV constructs for the miR-135a-5p sponge (S-135a) (upper panel) and a representative fluorescence image of the virus-infected slice (lower panel, n = 10). Bar = 1 mm. b qRT-PCR analyzes the expression of miR-135a-5p after injection of S-135a or scrambled sponge (Scr) to the hippocampus of C57 mice (n = 4 for each group). c–p The AAV packed miR-135a-5p sponge (S-135a) or scrambled control virus (Scr) were injected into the hippocampus of 6-month-old wild-type C57 mice. One month later, mice were subjected to the Morris water maze test (c–i), Barnes maze test (j–n) and fear conditioning test (o–p) to evaluate the learning and memory. c The representative traces to the hidden platform on day 6 of Morris water maze. d Latencies to the hidden platform in Morris water maze at learning stage were recorded (n = 10 for each group, repeated measures two-way ANOVA, p = 0.0087). e The representative traces on the probe trial at day 8. f–i Latencies to reach the previously hidden platform area (f), crossing times (g), total time spent in the different quadrants (h), and the swimming speed (i) on day 8 were analyzed (n = 10 for each group). j Latencies to the target hole for the first time were recorded during days 1–4 of the Barnes maze (n = 10 for each group, repeated measures two-way ANOVA, p = 0.0061). k The representative trace to reach the target hole on day 5. l Latencies to the target hole for the first time on day 5 (n = 10 for each group). m Time spent in nosing the target hole was evaluated (n = 10 for each group). n The accuracy of mice nosing target hole on day 5 (n = 10 for each group). o Percentage of freezing times was analyzed 24 h after contextual fear training (n = 10 for each group). p Percentage of freezing times was analyzed 48 h after fear training to tone (n = 10 for each group). (Data are presented as mean ± S.E.M. and two-tailed t tests were used unless otherwise specified. Source data are provided as a Source Data file. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Scr).

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