Fig. 6: Smc6-56-sup is defective in pathways that act complementarily with Top3 to prevent JM accumulation. | Nature Communications

Fig. 6: Smc6-56-sup is defective in pathways that act complementarily with Top3 to prevent JM accumulation.

From: Smc5/6 functions with Sgs1-Top3-Rmi1 to complete chromosome replication at natural pause sites

Fig. 6

a Additivity between smc6-56-sup and Top3 depletion induced by tetracycline (Tc) and auxin addition to Tc-top3-AID. b 2D gel of replication intermediates arising at TER302 in cells released synchronously from G1 in media containing 200 mM HU, tetracycline (Tc) and auxin. The experiment was repeated independently twice with similar results. Flow cytometry profiles are indicated on the right. Joint molecules accumulating on the X-arc are indicated by red arrows. Source data are provided as a Source Data file.

Back to article page