Fig. 1: SLAMF5 deficiency mitigates EAE and increases Breg levels.

EAE (MOG_35-55) was induced in WT and slamf5^−/− mice. Mice were followed for 26 days. a) Daily mean clinical scoring of the disease (WT group n = 23; slamf5^−/− group n = 15, two representative independent experiments out of seven similar experiments). b Change in mean body weight shown as percent of initial body weight (WT group n = 21; slamf5^−/− group n = 15, two representative independent experiments out of seven similar experiments). c, d On day 15, spleens, lymph nodes (LNs), and spinal cords were collected, leukocytes were incubated overnight with MOG_35-55 and analyzed by FACS for Th1 T cells (IL-17⁺ and RORγT⁺), Th17 T cells (IFNγ⁺ and T-bet⁺), and Treg (CD25⁺ FOXP3⁺) in (c) spleen (WT n = 5; slamf5^−/− n = 4, one experiment) (d) LNs, with gating for CD3⁺CD4⁺. (WT n = 9–10; slamf5^−/− n = 7–10, two independent experiments). e–m Four to five spinal cords were pooled together and analyzed for cytokine expression; showing representative histograms, percentage of secreting population, and cell count for: e–g IL-17⁺ (WT n = 5; slamf5^−/− n = 5, one experiment), h–j IFNγ⁺ T (WT n = 5; slamf5^−/− n = 4, one experiment), and k–m IL-10⁺ (WT n = 3; slamf5^−/− n = 3, one experiment) under the gate of CD45⁺CD11b⁻CD3⁺CD4⁺. Data expressed as mean ± s.e.m. (a, b) and mean ± s.d. (a, b insets, c, d, f, g, i, j, l, m). Mann–Whitney test (a, b insets). Unpaired Student’s t test with 95% confidence levels two-tailed (c, d, f, g, i, j, m), one-tailed (l). (***P < 0.001, ****P < 0.0001). Gating strategy for c and d is shown in Supplementary Fig. 1a(I.-IV.), gating strategy for e–m is shown in Supplementary Fig. 1g.