Fig. 4: SPA and LM-2I enhance ASS1 catalytic activity and its kinetics. | Nature Communications

Fig. 4: SPA and LM-2I enhance ASS1 catalytic activity and its kinetics.

From: Naturally-occurring spinosyn A and its derivatives function as argininosuccinate synthase activator and tumor inhibitor

Fig. 4

The recombinant ASS1 protein was incubated with different concentrations of SPA (a) or LM-2I (b) overnight, and the ASS1 catalytic activity was measured. The initial increase in absorbance at 660 nm (A660) between 1 and 8 min is expanded in the middle panel. In the right panel, the Hill 1 model of Origin 2018 software was used to analyze the increase in enzymatic reaction speed at different concentrations. The maximal activation ratio and AC50 were calculated. (mean ± s.d.). c The determination of kobs for the interaction of ASS1 (0.5 µM) with Biotin-SPA (20 µM) was calculated at different time points (n = 3 biologically independent experiments). d Plotting the kobs values for binding ASS1 (0.5 µM) with different concentrations of Biotin-SPA. BIAcore diagram (left panel) and KD (right panel) of the recombinant ASS1 protein binding to SPA (e) or LM-2I (f).Source data are provided as a Source Data file.

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