Fig. 1: PdmH1N1 infection induces infiltration of thymus-derived γδ T cells into lung.
a Representative plots (left) and frequency and number (right) of γδ T cells in lungs of pdmH1N1-infected mice (n = 9). b, c Mice at 1 dpi were i.v. transferred with or without 2 × 106 γδ T cells purified from mice at 4 dpi. b Kaplan–Meier survival rate (n = 9, 10). c Representative hematoxylin and eosin (H&E) histology (left) and scoring (right) of lungs from mice at 5 dpi (n = 3). Images are at original magnification ×200. Scale bar, 40 μm. d Kaplan–Meier survival rate of mice with i.p. injected 200 μg of anti-TCRγδ or control antibodies 3 days before infection (n = 10). e Schema of FTY720 injection (f) and thymectomy (ThX) surgery (g). f Flow cytometry plots (left) and cumulative data (right) showing frequencies and numbers of CD3+TCRγδ+ cells in gated live singlets from the indicated organs (n = 3, 4, 4). MedLN mediastinal lymph nodes, PleuralC pleural cavity, MLN mesenteric lymph nodes. g Mice received ThX or mock surgery 1 day before infection (n = 5). Representative plots (left) and cumulative data (right) showing the frequencies and numbers of CD3+TCRγδ+ cells from lungs at 4 dpi. h, i Mock-infected and pdmH1N1-infected mice at 5 dpi were i.v. injected with 40 μg of FITC-conjugated antibody to CD45 10 min before they were killed. Mice were perfused with PBS and tissues were harvested and analysed. Since MedLN is only detectable from 2 dpi onwards, and γδ T cells are only detectable in BLF of infected mice, analysis of γδ T cells in MedLN and BLF was conducted only with infected mice. Representative plots (h) and frequency pie charts (i) showing circulating γδ T (CD45+) or parenchyma-associated γδ T cells (CD45−) in gated CD3+TCRγδ+ cells (n = 5). Data are combined from two or three independent experiments and presented as mean ± SEM. P values were determined using two-tailed unpaired Student’s t-test (c, g, h), Gehan-Breslow-Wilcoxon Test (b, d) or one-way ANOVA (a, f). Source data are included in Source Data file.
