Fig. 3: Immune response under physiological conditions.
From: Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging
a Distinct changes in global behavior of antigen-presenting cells as measured by XCR1+ dendritic cell motility 24 h after immunization show the cell behavioral correlates of developing immune responses. (Left) Tracks of motility in control and 24 h post immunization, (right top) log histograms of motility coefficients, and (right bottom) displacement vs square root of time show that dendritic cells switch from faster random walk behavior in the control (i.e., straight line in bottom right plot) to slower, confined motility 24 h post immunization. b T cell motility at 24 h post immunization. (Top middle) log histograms of OT1, OT2, polyclonal T cells. (Top right) Displacement vs square root of time plots. (Bottom) tracks of T cell motility. c Dendritic cell clustering can be visualized and quantified on the whole lymph node level. (Top) 3D view with colored bars marking areas shown in 2D projections below. XY, YZ, XZ projections with zoomed-in area show an example of dendritic cell cluster forming over 26 min. (Bottom) Histograms of dendritic cell motility at 5 h post infection vs control, mean displacement vs square root of time, mean normalized density over time in 5 h post infection vs control dataset show that formation of dendritic cell clusters can be detected on the timescale of 1 h, but without any detectable change in dendritic cell motility. Error bars on all plots show 95% confidence intervals.
