Fig. 2: Effects of eucentric plane correction during tilt-series acquisition. | Nature Communications

Fig. 2: Effects of eucentric plane correction during tilt-series acquisition.

From: Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography

Fig. 2

a, b Comparison of the target displacement during tilting assuming that the sample is flat compared to the center of rotation (a, green arc) or when considering an off-plane shift (b, red arc and red lines). Note that the trajectory of an off-plane target is different than for an eucentric target. c Measurement of the median displacement in the x-coordinate (blue) and y-coordinate (red) without correcting for off-plane shift (left panel) and with correction (right-panel) over multiple tilt-series (n=150). Error bars represent the inter quartile range (IQR). d Measurement of the median defocus variation from the first image without correcting for off-plane shift (left panel) or with correction (right-panel) over multiple tilt-series (n = 150). Error bars represent the IQR. In general, errors in defocus originate mainly from errors in targeting and are not systematic.

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