Fig. 4: Self-tuning exposure weighting based on assigned particle scores. | Nature Communications

Fig. 4: Self-tuning exposure weighting based on assigned particle scores.

From: Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography

Fig. 4

a Average similarity scores calculated over individual particle projections and plotted as a function of the tilt-angle revealing the expected characteristic of the dose-symmetric scheme showing higher quality data obtained at the lower tilts. b 2D plot showing the cumulative contribution of individual tilts to the 3D reconstruction as a function of spatial frequency. Each colored band represents one tilt. The high frequency information is contributed mostly by lower tilts which are also exposed first.

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