Fig. 4: HDA complex mutants have extended lifespan and enhanced genotoxic resistance.

A RLS of WT, hda1Δ, hda2Δ, and hda3Δ measured by microfluidic method. B RLS of WT, hda1Δ, hda2Δ, and hda3Δ measured by manual microdissection. C Gene ontology biology process analysis on upregulated genes on hda1Δ transcriptome. Pathways with Benjamini p-value > 0.05 were selected. D Deletion strains of which transcriptomes have highest correlation coefficient with hda1Δ. Genes involved in DNA damage response (DDR) is labeled in red. E RLS of WT, hda1Δ, tup1Δ, and hda1Δ tup1Δ measured by microfluidic method. F Chronological lifespan (CLS) of WT, hda1Δ and tup1Δ. Error bars represent standard error (SEM) calculated from three biological replicates. G Drug sensitivity test on designated conditions. Control plate used here is synthetic complete (SC) agar plate. H Growth curve of WT and hda1Δ in synthetic complete (SC) media, over period of 34 h. Error bars represent SEM, n = 3. I Growth curve of WT and hda1Δ in synthetic complete (SC) media, with 0.03% MMS, over period of 34 h. Error bars represent SEM, n = 3. J Growth curve of WT and hda1Δ in synthetic complete (SC) media, with 0.05% MMS, over period of 34 h. Error bars represent SEM, n = 3.