Fig. 7: The lifespan phenotypes of HDA mutants are mediated by H3K18 acetylation. | Nature Communications

Fig. 7: The lifespan phenotypes of HDA mutants are mediated by H3K18 acetylation.

From: Inactivating histone deacetylase HDA promotes longevity by mobilizing trehalose metabolism

Fig. 7

A RLS of WT, hda1Δ, H3K9Q, and hda1Δ H3K9Q. B RLS of WT, hda1Δ, H3K14Q, and hda1Δ H3K14Q. C RLS of WT, hda1Δ, H3K23Q, and hda1Δ H3K23Q. D RLS of WT, hda1Δ, H3K27Q, and hda1Δ H3K27Q. E RLS of WT, hda1Δ, H3K18Q, and hda1Δ H3K18Q. F RLS of WT, hda1Δ, H3K18R, and hda1Δ H3K18R. G Metagene plot and heatmap of H3K18 acetylation on trehalose (left) and glycolysis (right) promoters as detected by ChIP- Seq. H ChIP-qPCR of Hda1-myc binding on TPS1, TPS2, TPS3, and ACT1 promoters, under designated time after MMS treatment. Error bars represent SEM, n = 3. p-value calculated by two-sided T- test. I RLS of WT, hda1Δ, spt3Δ, and hda1Δ spt3Δ. J Mechanism of HDA mediated suppression of trehalose metabolism and its effect on yeast aging. See also Figure S4.

Back to article page